To Pour or to Spread, This is the Question
Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates – in a...
View ArticleHelp: My Gal Promoter Doesn’t Work
Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but...
View ArticleAm I Damaging My E. coli by Spinning at High Speeds?
Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will...
View ArticleHow To Ask For Reagents
Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other...
View ArticleTo Pour or to Spread, This is the Question
Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates – in a...
View ArticleHelp: My Gal Promoter Doesn’t Work
Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but...
View ArticleAm I Damaging My E. coli by Spinning at High Speeds?
Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will...
View ArticleHow To Ask For Reagents
Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other...
View ArticleTo Pour or to Spread, This is the Question
Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates – in a...
View ArticleHelp: My Gal Promoter Doesn’t Work
Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but...
View ArticleAm I Damaging My E. coli by Spinning at High Speeds?
Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will...
View ArticleHow To Ask For Reagents
Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other...
View ArticleTo Pour or to Spread, This is the Question
Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates – in a...
View ArticleHelp: My Gal Promoter Doesn’t Work
Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but...
View ArticleAm I Damaging My E. coli by Spinning at High Speeds?
Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will...
View ArticleHow To Ask For Reagents
Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other...
View ArticleHelp: My Gal Promoter Doesn’t Work
Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but...
View ArticleAm I Damaging My E. coli by Spinning at High Speeds?
Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will...
View ArticleHow To Ask For Reagents
Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other...
View Article
